By Tanya E. S. Dahms, Kirk J. Czymmek
The objective of this quantity is to explain the newest advances in microscopic tools, together with built-in suggestions, as utilized to mycology. every one bankruptcy will supply a quick assessment of a specific microscopic technique with linked benefits and boundaries, the learn questions that may be thoroughly addressed utilizing those microscopic equipment, the way it has been effectively utilized to handle mycological study questions, together with aiding and complimentary options, and which destiny questions could be addressed.
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Extra resources for Advanced Microscopy in Mycology
W. Roberson Czymmek KJ, Fogg M, Powell DH, Sweigard J, Park SY, Kang S (2007) In vivo time-lapse documentation using confocal and multi-photon microscopy reveals the mechanisms of invasion into the Arabidopsis root vascular system by Fusarium oxysporum. Fungal Genet Biol 44:1011–1023 Day RD, Davidson MW (2009) The fluorescent protein palette: tools for cellular imaging. Chem Soc Rev 38:2887–2921. 1039/B901966A Delgado-Álvarez DL, Callejas-Negrete OA, Gómez N, Freitag M, Roberson RW, Smith LG, Mouriño-Pérez RR (2010) Visualization of F-actin localization and dynamics with live cell markers in Neurospora crassa.
2008), key players in the control of fungal development (Bayram et al. 2010; RodriguezRomero et al. 2010). 1c) . The BiFC technique has also been shown to be a valuable tool for the study of the interaction between apically located regulators of fungal development. The transcription factor FlbB interacts with the small filamentous fungal-specific protein FlbE (Garzia et al. 1d; Garzia et al. 2 Fluorescence-Based Methods for the Study of Protein … 31 2009). The apical localization of both proteins, which depends on this interaction, is essential to induce conidiophore development (asexual reproduction).
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Advanced Microscopy in Mycology by Tanya E. S. Dahms, Kirk J. Czymmek